Tight junction biogenesis during early development
نویسندگان
چکیده
منابع مشابه
Tight junction biogenesis in the early Xenopus embryo
Tight junctions (TJs) perform a critical role in the transport functions and morphogenetic activity of the primary epithelium formed during Xenopus cleavage. Biogenesis of these junctions was studied by immunolocalization of TJ-associated proteins (cingulin, ZO-1 and occludin) and by an in vivo biotin diffusion assay. Using fertilized eggs synchronized during the first division cycle, we found ...
متن کاملLocalisation of tight junction protein cingulin is temporally and spatially regulated during early mouse development.
The molecular maturation of the tight junction in the mouse early embryo has been investigated by monitoring the distribution of cingulin, a 140 x 10(3) M(r) peripheral (cytoplasmic) membrane constituent of the junction, at different stages of development and in different experimental situations. Although tight junction formation does not begin until compaction at the 8-cell stage, cingulin is ...
متن کاملTight junction protein cingulin is expressed by maternal and embryonic genomes during early mouse development.
The expression of the tight junction peripheral membrane protein, cingulin (140 x 10(3) M(r), was investigated in mouse eggs and staged preimplantation embryos by immunoblotting and immunoprecipitation. Polyclonal antibody to chicken brush cingulin detected a single 140 x 10(3) M(r) protein in immunoblots of unfertilised eggs and all preimplantation stages. Relative protein levels were high in ...
متن کاملTranscription factor AP-2γ is a core regulator of tight junction biogenesis and cavity formation during mouse early embryogenesis.
The trophectoderm epithelium is the first differentiated cell layer to arise during mammalian development. Blastocyst formation requires the proper expression and localization of tight junction, polarity, ion gradient and H2O channel proteins in the outer cell membranes. However, the underlying transcriptional mechanisms that control their expression are largely unknown. Here, we report that tr...
متن کاملGene expression regulating epithelial intercellular junction biogenesis during human blastocyst development in vitro.
We investigated gene expression associated with trophectoderm epithelial intercellular junction formation in single human embryos at different stages of cleavage using RT-PCR methods based upon magnetic bead separation of polyA+ RNA. Trophectoderm tight junction (TJ) and desmosome biogenesis contribute to intercellular sealing and tissue integrity, critical for vectorial transport and blastocoe...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2008
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2007.09.031